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Thi Thuy Duong, Sabine Ja ¨hnichen, Thi Phuong Quynh Le, Cuong Tu Ho, Trung Kien Hoang, Trung Kien Nguyen, Thi Nguyet Vu, Dinh Kim Dang (2014).

The occurrence of cyanobacteria and microcystins in the HoanKiem Lake and the Nui Coc reservoir (North Vietnam)Environmental Earth Sciences 71(5), pp. 2419–2427, DOI 10.1007/s12665-013-2642-2


Abstracts
The occurrence of increasing blooms of toxic cyanobacteria in freshwaters has received much attentiondue to the ability of many cyanobacteria to produce potent cyanotoxins. In this paper, the occurrence of dominantcyanobacteria and the concentration of microcystins (MCs) analysis were investigated monthly from July 2008 to April 2009 in the HoanKiem Lake and from February to April 2009 in the Nui Coc reservoir. Concentrations of intra-cellular MCs from water, bloom samples, and isolated strains were quantified by using high performance liquid chromatography (HPLC). During the study period, the microscopic examination of the phytoplankton samples showed the dominance of the genus Microcystis in the water environment of the HoanKiem Lake and the Nui Coc reservoir. The toxin analysis by HPLC demonstrated the presence of two MC variants: MC-LR and MC-RR in water samples. Total concentrations of the toxins in filtered samples from surface water ranged from non-detected to 0.91 lg L-1 at Nui Coc reservoir and they ranged from 2.1 to 46.0 lg L-1 at HoanKiem Lake. The results of the HPLC analysis confirmed the production of MCs in bloom samples (ranged from 115.9 to 184.6 lg L-1 in the HoanKiem Lake and from 726.5 to 1116 lg L-1 in the Nui Coc reservoir) and isolated strains of Anabaena sp. and Microcystis with the concentration of MC ranging from 152 to 396.2 lg g-1 dry mass, respectively.
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